IngentaConnect Organogenesis from megagametophyte and zygotic embryo explants of...

Authors: Chavez V.M.¹; Litz R.E.²

Source: Plant Cell, Tissue and Organ Culture, Volume 58, Number 3, 1999 , pp. 219-222(4)

Publisher: Springer

Abstract:

Organogenic cultures were induced from zygotic embryo and megagametophyte explants of the Central American cycad species, Dioon edule. Plant growth medium consisted of B5 major salts, Murashige and Skoog minor salts and organics, 400 mg l^-1 glutamine, 100 mg l^-1 arginine, 100 mg l^-1 asparagine, 60 g l^-1 sucrose, 8 g l^-1 Difco Bacto agar and was supplemented with kinetin (0 – 13.94 M) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0 – 9.05 M) arranged as a 5×4 factorial in a randomized block design. Callus initiation occurred on a wide range of medium formulations from megagametophyte explants; however, shoot formation occurred only on medium supplemented with 2.26 M 2,4-D. In comparison, callus initiation from explanted zygotic embryos occurred on fewer medium formulations, and adventitious shoot induction occurred from callus on formulations with 9.29–13.94 M kinetin + 0.45–9.05 M 2,4-D. Rooted shoots, derived from megagametophyte and zygotic embryo cultures, have been regenerated.

Keywords: cycad; gymnosperm; organogenesis; megagametophyte; in vitro; Dioon edule

Language: English

Document Type: Regular paper

Affiliations: 1: Jardin Botanico, Instituto de Biologia, Universidad Nacional Autonoma de Mexico, Mexico DF 04510, Mexico 2: Tropical Research and Education Center, University of Florida, 18905 SW 280 St., Homestead, FL 33031-3314, USA requests for offprints: Tel: +1 305 246 7017; Fax: 305 246 7003; E-mail: rel@gnv.ifas.ufl.edu

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Organogenesis from megagametophyte and zygotic embryo explants of the gymnosperm Dioon edule Lindley (Zamiaceae, Cycadales)